By Dr. Norbert Klöpper, Product Manager, Bruker Daltonics, Bruker Daltonics Inc.
SubscribeAPSIS is an identifier for harmful pathogens, like bacteria, spores and viruses, based on their genomic fingerprint stored in their DNA or RNA. It can be used for identification of Biological Warfare Agents (BWA) as well.
The principle of Polymerase Chain Reaction (PCR) for amplification of the sample's DNA or RNA is used and the subsequent hybridization against specific probes allows controlling even modified species.
By combining the power of DNA amplification by PCR (Polymerase Chain Reaction) with the state of the art DNA microarray technology into one cartridge a unique, reliable system was created.
APSIS provides the entire processing facility to unleash the power of the integrated cartridge with minimal user interaction.
Key features:
- thin film heater combined with temperature sensor attached to the microarray chip to perform PCR
- amplification products are hybridized against the specific probes in the same chamber
- PCR and Hybridization are performed in presence of the same buffer
- Increased accuracy by combination of two specific technologies (PCR and Hybridization) yields to reliable results
- EPROM for internal data storage gives you an increased process visibility, control and processing flexibility
- Parallel but independent thermal processing of up to eight cartridges
- Software based image analysis with reports
APSIS is a platform concept that makes DNA based testing as easy as today's pregnancy testing.
First available applications:
- Detection of pathogens via PCR amplification of species-specific DNA markers
- Combined Anthrax/Smallpox Screening
- Verification of infection with human brain viruses in brain liquor